Skeletal muscle differentiation of human iPSCs meets ... - Nature
WEBApr 7, 2022 · In vivo, skeletal muscles can self-regenerate: after traumas or other tissue damage, resident muscle stem cells named muscle satellite cells (MuSCs), are activated.
iPSC s: A powerful tool for skeletal muscle tissue engineering
WEBApr 1, 2019 · Satellite cells (so called because of their peripheral location) are a population of muscle stem cells residing between basal lamina and sarcolemma. These cells can either differentiate and proliferate, promoting muscle growth and regeneration or self‐renew to maintain the satellite cells pool.
Large-Scale Expansion of Human iPSC-Derived Skeletal Muscle Cells …
WEBJun 5, 2018 · Muscle regeneration is mediated by satellite cells (SCs) (Lepper et al., 2011; Murphy et al., 2011; Sambasivan et al., 2011); i.e., adult stem cells located between the sarcolemma and the plasma membrane (Mauro, 1961) that are quiescent in healthy, uninjured muscle.
Regenerating human skeletal muscle forms an emerging niche in …
WEBNov 2, 2023 · We hypothesized competition with mouse satellite cells (SCs) prevented SMPC engraftment into the SC niche and thus generated an SC ablation mouse compatible with human engraftment.
Pluripotent stem cell-induced skeletal muscle progenitor cells …
WEBFeb 12, 2021 · Satellite cells (SCs) are endogenous skeletal muscle stem cells, which are responsible for muscle maintenance and muscle regeneration after injury [1, 2]. A previous study reported that xenotransplantation of human SCs into mice achieved efficient engraftment and populated the satellite niche [ 3 ].
Functional skeletal muscle model derived from SOD1-mutant …
WEBAug 31, 2020 · This patient iPSC-derived muscle model is human-based, free of other cell types such as motoneurons, and allows the investigation of muscle pathology from myogenesis to functional muscle...
Novel human iPSC models of neuroinflammation in …
WEB1 day ago · Several co-cultures of iPSC-derived CNS cells can be reproduced in vitro (dashed arrows) [12,91,93]. In this review, we discuss the latest research, primarily focusing on the progress made over the past 5 years in the design and application of hiPSC models to study neuroinflammation. These models should complement mechanistic animal studies.
Three-Dimensional Human iPSC-Derived Artificial Skeletal ... - Cell …
WEBApr 17, 2018 · Here, we report the generation of three-dimensional (3D) artificial skeletal muscle tissue from human pluripotent stem cells, including induced pluripotent stem cells (iPSCs) from patients with Duchenne, limb-girdle, and congenital muscular dystrophies. 3D skeletal myogenic differentiation of pluripotent cells was induced within hydrogels under ...
Differentiation of the human PAX7-positive myogenic precursors ...
WEBJun 26, 2020 · Here, we report the generation of human induced pluripotent stem cell (iPSC) reporter lines in which fluorescent proteins have been introduced into the PAX7 and MYOG loci. We use single cell RNA sequencing to analyze the developmental trajectory of the iPSC-derived PAX7 + myogenic precursors.
Myogenic Differentiation of Stem Cells for Skeletal Muscle …
WEBFeb 8, 2021 · Darabi et al. introduced Pax7 into human ES and iPSC and found that it not only produces a large number of induced myogenic progenitors (iMPCs) with regenerative ability but also contributes to the satellite cell pool and maintains it for a long time after implantation in animals . Culturing iMPC in a 2D environment, it will differentiate into ...
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