Skeletal Muscle Satellite Cells: Background and Methods for …
WEBSatellite cells were initially described fifty years ago by their anatomical location on the surface of muscle fibers, between the myofiber plasmalemma and the basal lamina (4, 5) (for a schematic and electron microscope image see Fig. 1).
Satellite Cells and the Muscle Stem Cell Niche - PMC
WEBSatellite cells can be identified by fluorescence microscopy via combined immunofluorescence labeling of laminin and M-cadherin, which respectively label the basal lamina and plasma membrane of satellite cells contacting the myofibers .
Skeletal Muscle Satellite Cells: Background and Methods for …
WEBJan 1, 2011 · Repair of adult skeletal muscle depends on satellite cells, myogenic stem cells located between the basal lamina and the plasmalemma of the myofiber. Standardized protocols for the isolation and culture of satellite cells are key tools for understanding cell...
Human satellite cells: identification on human muscle fibres
WEBSatellite cells, normally quiescent underneath the myofibre basal lamina, are skeletal muscle stem cells responsible for postnatal muscle growth, repair and regeneration.
Intravital microscopy of satellite cell dynamics and their …
WEBOct 18, 2023 · Using a homebuilt dual-laser nonlinear optical microscope platform, we simultaneously visualized various cell types and both intracellular and extracellular structures in the skeletal muscle, including RFP + monocytes/macrophages, RFP − nonmyogenic cells, YFP + MuSCs/ASCs, collagen, and muscle fibers, which facilitates the examination of ...
The Skeletal Muscle Satellite Cell: The Stem Cell That Came in …
WEBMore recently, several molecular markers have been described that can be used to detect satellite cells, making them more accessible for study at the light microscope level. Satellite cells supply myonuclei to growing myofibers before becoming mitotically quiescent in muscle as it matures.
Fate decision of satellite cell differentiation and self ... - Nature
WEBJul 22, 2019 · Reports have demonstrated that elevated JAK–STAT3 signaling in aging or diseased SCs leads to cells preferentially undergoing asymmetric cell fate segregation, resulting in impaired muscle ...
The skeletal muscle satellite cell: the stem cell that came in …
WEBThe muscle satellite cell was first described and actually named on the basis of its anatomic location under the basement membrane surrounding each myofiber. For many years following its discovery, electron microscopy provided …
The muscle satellite cell at 50: the formative years
WEBAug 17, 2011 · The journey of the satellite cell from electron microscope curio, to its gradual acceptance as a bona fide myoblast precursor, is then charted: work that provided the foundations for our understanding of the role of the satellite cell.
Satellite cells in human skeletal muscle plasticity - Frontiers
WEBOct 21, 2015 · Skeletal muscle satellite cells are considered to play a crucial role in muscle fiber maintenance, repair and remodeling. Our knowledge of the role of satellite cells in muscle fiber adaptation has traditionally relied on in vitro cell and in vivo animal models.