Skeletal Muscle Satellite Cells: Background and Methods for …
WEBAlternatively, satellite cells can be enriched from whole muscle cell suspensions by various approaches that reduce the presence of fibroblastic cells, typically present to some degree in the preparation, and remove myofibril debris present in the initial cell suspension.
Isolation and Purification of Satellite Cells for Skeletal Muscle ...
WEBUsing this approach, muscle tissue is dissected and dissociated into a single cell suspension. Initially, all visible tendon and connective tissue is removed from the harvested muscle to maximize final myogenic purity.
A novel approach for the isolation and long-term expansion of …
WEBMar 17, 2021 · Satellite cells (SCs) are muscle stem cells capable of regenerating injured muscle. The study of their functional potential depends on the availability of methods for the isolation and expansion of pure SCs with preserved myogenic properties after serial passages in vitro.
Isolation of satellite cells and transplantation into mice for …
WEBJan 22, 2020 · Satellite cell heterogeneity has a significant role in muscle regeneration, where the satellite stem cells maintain muscle stem cell homeostasis by undergoing asymmetric division to give rise...
Purification and preservation of satellite cells from human skeletal ...
WEBMar 3, 2021 · Regeneration and repair of skeletal muscle is driven by tissue-specific progenitor cells called satellite cells, which occupy a minority of the cells in the muscle. This protocol provides researchers with techniques to efficiently isolate and purify functional satellite cells from human muscle tissue.
Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle
WEBFeb 23, 2017 · Satellite cells play a pivotal role in muscle homeostasis and are the major source of myogenic precursors in mammalian muscle regeneration. This chapter describes protocols for isolation and culturing satellite cells isolated from mouse skeletal muscles.
Skeletal muscle satellite cells: background and methods for
WEBThis chapter provides an introduction to satellite cell biology and further describes the basic protocol used in our laboratory to isolate and culture satellite cells from adult skeletal muscle.
Fate decision of satellite cell differentiation and self ... - Nature
WEBJul 22, 2019 · Quiescent satellite cells (SCs) that are activated to produce numerous myoblasts underpin the complete healing of damaged skeletal muscle. How cell-autonomous regulatory mechanisms modulate...
Satellite cell depletion does not inhibit adult skeletal muscle ...
WEBOct 15, 2012 · satellite cells are resident muscle stem cells located between the sarcolemma and the myofiber basal lamina. Following postnatal development they remain largely quiescent under resting conditions, but become activated in response to muscle injury, stretch, and mechanical load ( 29 ).
Isolation and Culture of Quiescent Skeletal Muscle Satellite Cells
WEBMay 31, 2020 · It has been shown that freshly isolated satellite cells from adult muscle constitute a stem cell-like population that exhibits more efficient engraftment and self-renewal activity in regenerating muscle than myoblast. Thus, purification of …