RevIT AAV Enhancer increased genome titers 1.7- to 2.2-fold with other transfection reagents compared to their respective controls, demonstrating broad applicability. Figure 1.

This schematic shows the steps involved in generating recombinant AAV by the triple-transfection method using plasmid DNA. The application of the cell-based qPCR assay at SK pharmteco has proven ...

The system achieves high AAV titers through high-density suspension culture of a clonal 293F-derived cell line in chemically defined, animal origin free medium. Helper-free triple transfection is ...

But the triple transfection of the required plasmids, and in the strict stoichiometries required for AAV assembly, we believe adds enormously to the cost of traditional AAV manufacturing, limiting ...

So, the classical standard for AAV, triple plasmid transient transfection, does not do the job? Correct. Transient transfection of cells using plasmids works well in smaller volumes and academic ...

TruStable™ production cell line produced ~15-fold more AAV with ~3-fold higher packaging percentage than the comparator triple transfection system, resulting in ~30-fold more material generated ...

Fig 1. Vector genome maps for plasmids used in the AAV triple plasmid transfection assay. AAV, adeno-associated viruses; eGFP, enhanced green fluorescent protein; GOI, gene of interest; ITR ...

Agathos produced rAAV with a transgene sequence provided by Genovac in AE1-BHK using transfection with three plasmids (triple transfection), a well-established process that has been used ...

Adeno-associated virus (AAV) vectors are a versatile and appealing gene therapy delivery platform, capable of targeting a wide range of cell types. However, AAV must be produced in a living system, ...