Fill the Eppendorf tube with 1 ml of degassed deionized water. ... A time-lapse sequence showing how the lipid at the source is extruded into membrane tubes in response to buffer flow.

Resuspend the mitochondrial pellet (from Step 31) in 0.5 ml HDP buffer in a 1.5-ml snap-cap microfuge tube; if 0.5 ml is less than 10 volumes, split the pellet into multiple tubes. 45 Incubate on ...