Further study was conducted by using FIT fed-batch media (synthetic and complex formulation) both in Duran baffled flasks and Duran Erlenmeyer flasks.

Transfer the reaction mixture to an Erlenmeyer flask and wash the Duran bottle twice with ∼ 20 ml of Et 2 O. Swirl the bottle several times so that any product remaining between the tubings is ...

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We were able to show that the Duran baffled flask exceeds the classical Duran Erlenmeyer flask in oxygentransfer capacity by more than 50%, enabling a better aeration for aerobic cultures.