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Many doubted the GFP gene would produce the glowing protein on its own. But when Chalfie put it in bacteria and shined a blue light on them, they glowed. Chalfie's 1994 paper on the gene ...
GFP has been recognized as a marker in intact cells for gene expression and protein targeting. In biological studies, it is extensively used as genetically encoded fluorescent markers.
But first, the gene that creates the GFP protein needed to be found. William Ward, a professor at Rutgers University, met Douglas Prasher on a jellyfish-hunting expedition in the 1980s.
A green fluorescent protein makes fruit fly sperm glow green. (Image credit: Catherine Fernandez and Jerry Coyne.) GFP remains special, however, because it spontaneously folds into the right shape ...
A transgenic line of monkeys carrying a gene encoding green fluorescent protein fully integrated into their DNA has been created for the first time. The research, published in the journal Nature ...
Since Prasher cut the GFP gene out of the jellyfish genome with restriction digestion enzymes, the clone had extra DNA segments that bookended the GFP gene. If Chalfie and Euskirchen wanted only the ...
The best gene tested was mGFP5er, a mutagenized GFP gene that is targeted to the endoplasmic reticulum. We also demonstrated that host plants synthesizing GFP in the field suffered no fitness costs.
In this system, base-editing activity results in the expression of green fluorescent protein (GFP). The team installed a mutation in the bacterium's GFP gene to remove its fluorescence.
Fluorescent jellyfish gene sheds light on 'fitness landscape' Date: May 12, 2016 Source: Centre for Genomic Regulation Summary: By studying more than 50,000 variants of a jellyfish gene ...
The researchers still have more work to do to determine whether the anti-FIV gene works in the cats. “We haven’t shown cats that are AIDS-proof,” study co-author Eric Poeschla told LiveScience .