GST–EB1 was used to precipitate GFP–MCAK from cells treated with the MT depolymerizing drug nocodazole under conditions known to destroy essentially all cytoplasmic MTs (Morrison et al., 1998 ...

Expression and purification of GST-VCY fusion protein. GST-VCY fusions were expressed in E.coli strain BL21 (DE3). The molecular weight of the fusion protein was ∼ 43 kD (Fig 2, lane 4 ...