The roGFP fluorescence (490–560 nm) was detected by a bialkali-cathode PMT and a Dodt contrast-detector system that provided a bright-field transmission image (Prairie Technologies).

At Taos, Surmeier outlined results published late last year in Nature based on targeting the roGFP reporter to the mitochondrial matrix of dopaminergic neurons. He found that calcium entry through ...

The cover shows bioimaging of mouse liver oxidative stress after partial hepatectomy. roGFP (reduction/oxidation-sensitive green fluorescent protein) allows real-time visualization of the ...

The roGFP also included a sequence that targeted the protein to mitochondria. Since roGFP lights up when oxidized, the protein provided a way to directly visualize the oxidation state of mitochondria.

Redox processes play an important role in cellular physiologyand pathology. A particularly powerful tool for the monitoringof cellular redox changes are genetically-encoded biosensorsbased on redox ...