First, a BAC library was screened using primers derived from sequencing of YAC D9 subclones. Of the eight BACs identified, BAC 508K21 was found to overlap most with YAC D9 and was used as driver ...

(a) BAC/PAC/YAC plasmids can be delivered through transfection reagents such as PEI. 1–2: The AC is purified and mixed with a suitable lipid-based biochemical to form DNA complexes. 3: Target ...