Resuspend the mitochondrial pellet (from Step 31) in 0.5 ml HDP buffer in a 1.5-ml snap-cap microfuge tube; if 0.5 ml is less than 10 volumes, split the pellet into multiple tubes. 45 Incubate on ...

Fill the electrode chambers with the appropriate electrode buffer. 10| Push the IEF gels from the glass tubes, one at a time, into a small tray of equilibration buffer. As the tube emerges, ...