In the current study, the scientists installed up to five distinct mutations on a single lambda phage genome without counterselection in only a few hours of hands-on time.

Previous research had shown that the lambda phage introduced its own genetic information into that of its host, inserting it at a specific site in the host genome. ... the 3D structure ...

In collaboration with colleagues from Forschungszentrum Jülich and Jena University Hospital, they have now revealed the 3D structure of this crucial phage component in atomic resolution.

The lambda phage, for example, prefers to destroy E. coli bacteria, ... In the lysogenic cycle, the phage's DNA lives as part of the bacterium itself and both continue to reproduce as one.

In this study, they found that the lambda phage shares this mechanism. If the DNA slips to the extent that it would completely fall out, it is caught by its end and prevented from detaching from ...

Scientists have now revealed the 3D structure of a phage component in atomic resolution, by combining two methods - solid-state NMR and cryo-electron microscopy.

During this time, the phage genome would seem to be vulnerable to any antiviral enzymes floating around the host cell. But somehow, the genome remains intact while the safe room is under construction.

Scientists have now revealed the 3D structure of a phage component in atomic resolution, by combining two methods - solid-state NMR and cryo-electron microscopy.