Increased Transfection Efficiency Boosts AAV Titers for Gene Therapies by 2–4X Mirus Bio describes an enhancer that is simple to use, easily integrates into existing workflows, and produces high ...

The triple-transfection method is efficient and scalable, enabling the production of various AAV serotypes by altering the cap gene within the rep-cap plasmid.

AAV is manufactured by the triple transfection of three plasmid DNA constructs into packaging cell lines to produce recombinant adeno-associated virus.

In this talk, we present optimization strategies for the transfection step of vector production to generate high titers of both AAV and LV using TransIT-VirusGEN® Transfection Reagent. The VirusGEN® ...

Material and methods A commercial vendor received two AAV samples, one serotype 5 (AAV5) and one serotype 9 (AAV9). The two samples were generated via the triple plasmid transfection approach.

TruStable™ production cell line produced ~15-fold more AAV with ~3-fold higher packaging percentage than the comparator triple transfection system, resulting in ~30-fold more material generated ...

Agathos produced rAAV with a transgene sequence provided by Genovac in AE1-BHK using transfection with three plasmids (triple transfection), a well-established process that has been used ...

This work studied the impact of the transgene size on AAV production, purification, and stability. AAV2-GFP was produced using suspension adapted HEK293 cells via triple transfection with three ...

AAV is manufactured by the triple transfection of three plasmid DNA constructs into packaging cell lines to produce recombinant adeno-associated virus.