Western blotting of cell lysates was carried out as previously described. 23 Cell lysates ... and then incubated with normal mouse serum for five minutes. Finally, CD3–phycoerythrin, CD56 ...

What is western blotting? In 1975, Southern invented a new method that enabled analysis of DNA identity, size and abundance. It involved the use of electrophoresis to separate DNA fragments based on ...

Western blot analysis was performed and immunoblotting with anti-CD3 zeta monoclonal antibody produced bands of predicted size (57.86 kDa) for the CD5CAR-CD3 zeta fusion protein .