In many labs, you will see Erlenmeyer flasks used for shake cultures filled to 50% of the nominal volume, which is not a good idea, considering that most cultures are supposed to be aerobic ones.

Mix 175g of NaOAc trihydrate with 50mL distilled water in a very clean 500mL erlenmeyer flask and heat in a boiling water bath until all solid is dissolved. Remove from bath, rinse down sides with ...