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Protein crystallization and phase diagrams - ScienceDirect
Nov 1, 2004 · In this article, methods to determine the phase diagram of an aqueous solution of a globular protein are described, focusing on the solid (crystal) and condensed liquid states. The …
- Author: Neer Asherie
- Publish Year: 2004
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Protein folding and surface interaction phase diagrams
This review discusses three aspects of protein phase diagrams. First, the relevance of phase diagrams to protein folding and function in vitro and in cells. Next, how the evolution of protein …
Goal: Understand the general phenomenology of phase transitions and phase coexistence conditions for a single component system. The Chemical Potential µ controls phase transitions …
Determination of Protein Phase Diagrams by Centrifugation
Microscopy and turbidity measurements can be used to determine the concentration of a protein above which it phase separates, the so-called saturation concentration. Here, we describe …
- Author: Nicole M. Milkovic, Tanja Mittag
- Publish Year: 2020
8.2: Single Component Phase Diagrams - Chemistry …
In this diagram, the phase boundaries can be determined by measuring the rate of cooling at constant temperature. A typical cooling curve is shown below. The temperature will decrease over time as a sample is allowed to cool.
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One component phase diagram 2 Chapter 9: Phase diagrams Two component phase diagram. 2 3 Chapter 9: Phase diagrams 0 20 40 80 100 1000 1100 1200 1300 1400 1500 1600 T(°C) 60 …
Designer protein assemblies with tunable phase …
Jul 13, 2020 · A synthetic phase separation system consisting of two protein components with tunable parameters was developed to visualize and characterize phase diagrams in living cells, revealing that...
Understanding protein crystallization on the basis of the phase …
Jan 15, 1999 · The phase diagram of a protein–water system is described with a simple model with parameters for the interaction between the protein molecules in the crystal and in the …
Jun 4, 2020 · To address this challenge, we present here a combinatorial droplet microfluidic platform, termed PhaseScan, for the rapid and high-resolution acquisition of protein phase …
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